Volume 12, Number 1, January 2006
SARS–associated Coronavirus Replication in Cell Lines
Matthew Kaye,* Julian Druce,* Thomas Tran,* Renata Kostecki,* Doris
Chibo,* Jessica Morris,* Mike Catton,* and Chris Birch*
*Victorian Infectious Diseases Reference Laboratory, North Melbourne,
Victoria, Australia
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Figure 2. Cycle threshold (Ct) changes measured by real-time polymerase chain reaction versus days after infection of the indicated cell lines. The cell-free sample had an initial Ct of 31, which rose to 40 by day 11. Reductions in the Ct or flat-line Ct values (e.g., COS cells) indicate replication of the virus. Continued increases in Ct above the initial value of 31 by days 7 and 11 indicate failure to replicate. |
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